The three strategies to construct HAC have been explored without full knowledge of chromosome elements such as centromere, telomere and replication origin.

本文探讨了在不完全了解着丝粒、复制起始点、端粒等人类染色体基本功能单位的情况下构建HAC的三种策略。

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The vector is in ring shape , comprising a procaryon replication origin, two eucaryon replication origins and selective marker genes, and two green fluorescent protein gene expression cassettes;

该载 体是一种环状载体，包含原核复制起点、真核复制起点、筛选标记基因和绿色荧光蛋 白基因表达盒；

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In vitro DNA replication system derived from eukaryotes is capable of replicating exogenous plasmid DNA containing SV40 origin of replication efficiently and accurately.

报道了含sv40复制起点的质粒dna在真核细胞抽提物中进行复制的DNA体外复制系统的建立。

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It has to have an origin of replication, which we talked about last time, which is compatible with the cells.

所以质粒必须带有复制起点,我们上次讲过,该起点要与受体细胞匹配

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So having a place that the cell knows - where the cell knows how to replicate is important and so plasmids have an origin of replication.

所以得有一个细胞能识别的区域,能使细胞知道,怎样来复制 这很重要,因此质粒上带有一个复制起点

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This region here of this particular plasmid is called the ori or origin of replication.

质粒上的这个区域叫,ori,或者叫复制起点

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